The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate coordinated motor pattern in Caenorhabditis elegans
Fig 8
GOT-1 is not a direct substrate of UBR-1.
A) The total GOT transaminase activity was increased in ubr-1 and ubr-1got-2 mutants, but decreased in got-1 and ubr-1; got-1 mutants. *P<0.05; **P<0.01 by the Kruskal-Wallis test. Horizontal lines represent the mean values. B) The loss of ubr-1 did not lead to changes in GOT-1::GFP protein levels in C. elegans carrying endogenously fused GOT-1::GFP (left panel) or GOT-1::GFP driven by an exogenous pan-neuronal promoter (right panel). C) A working model of UBR-1-mediated negative regulation of glutamate level and bending.