The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate coordinated motor pattern in Caenorhabditis elegans
Fig 2
A critical requirement of UBR-1 in premotor interneurons during reversals.
A) The UBR-1::GFP reporter was expressed in a subset of neurons including the premotor interneurons of the reversal circuit (Premotor INs), the pharyngeal muscles, the body wall muscles, and hypodermal seam cells. Scale bar 10μm. B) Reduced bending in ubr-1 mutants (grey line) was rescued by restoring UBR-1 expression in neurons (red line), but not in muscles (blue line). C) Reduced bending in ubr-1 mutants (grey line) was robustly rescued by restoring UBR-1 expression in glutamate-receptor-expressing, premotor interneurons (INs), not in cholinergic or GABAergic motor neurons (MNs). D) Among the premotor interneurons (INs), a robust rescue required UBR-1 expression in AVE and RIM (red line). *P<0.05, ** P<0.01, ***P<0.001 by Two-way RM ANOVA. Data are represented as mean ± SEM.