DNA strand-exchange patterns associated with double-strand break-induced and spontaneous mitotic crossovers in Saccharomyces cerevisiae
Fig 2
Crossover-specific assay for hetDNA mapping.
The recipient lys2Δ5′ allele (white box) on chromosome II contains an in-frame I-SceI cut site (arrowheads indicate corresponding nicks). The lys2Δ3′ allele (black box) on chromosome V is also in frame and contains an I-SceI cut site inactivated by a 6-bp insertion (boxed). The nearest flanking SNPs (red) are 8 bp from each 3′ end of the I-SceI generated DSB. Only a CO event can reconstitute a full-length LYS2 allele and both recombination products must co-segregate for Lys+ colony formation. hetDNA flanking the initiating DSB is indicated as gray boxes, and was monitored in each product by SMRT sequencing. The product-specific primers for PCR amplification are indicated by black horizontal arrows.