A Caenorhabditis elegans protein with a PRDM9-like SET domain localizes to chromatin-associated foci and promotes spermatocyte gene expression, sperm production and fertility
Fig 5
SET-17 is expressed in the nuclei of primary spermatocytes and enriched in chromatin-associated foci.
A) Confocal imaging of a longitudinal section showing SET-17::GFP expression in germ cells and spermatocytes of an adult male germline. White lines delineate the gonad. Scale bar, 10 μm. A, anterior; P, posterior; D, dorsal; V, ventral. B) Representative Z-axis projection of confocal images of extruded and formaldehyde-fixed male germlines expressing SET-17::GFP and stained for GFP by immunofluorescence (yellow). DNA stained with DAPI (blue). Scale bar, 10 μm. Inserts, higher magnification views of individual representative nuclei. Scale bar, 1 μm. C) & D) Representative Z-axis projection of primary spermatocyte nuclei of males expressing SET-17::GFP. Nuclei were stained by immunofluorescence for GFP and H3K4me1 C) or H3K4me2 D), respectively. DNA, DAPI. Scale bar, 1 μm.