PIKfyve regulates melanosome biogenesis
Fig 2
Melanocyte specific PIKfyve knockout mice exhibit hair greying.
A) Timeline of in vivo experiments. Five Tyrosinase::CreERT2; PIKfyveFlox/Flox and 5 PIKfyveFlox/Flox mice were administered tamoxifen chow for 29 days. A control group of 5 Tyrosinase::CreERT2; PIKfyveFlox/Flox mice were fed a control diet throughout the course of the experiment. All mice were shave depilated at p50 and subsequently fed normal chow beginning at p50 for the subsequent days. Gray bar denotes the duration that mice, with the exception of the control group, were on tamoxifen feed. B) Littermates were photographed at P85, P105, and P365. C) Mouse hair from the genotypes indicated was dissolved in solune-350 and melanin quantitation was performed as described. The relative amount of melanin in the hair was calculated relative to Cre- controls at P85, P105,and P365. Data shown are mean ± S.D. (n = 5 or 3 as indicated by error bars. ***, p < 0.001 using a two-tailed Student’s paired T test.