The C-terminal of CASY-1/Calsyntenin regulates GABAergic synaptic transmission at the Caenorhabditis elegans neuromuscular junction
Fig 5
CASY-1 isoforms shows differential spatial localization.
(A) Expression of GFP under isoform-specific casy-1 promoters. casy-1a transcriptional reporter does not co-localize with mCherry marked cholinergic or GABAergic motor neurons. casy-1b and casy-1c expression reporters show expression in both cholinergic and GABAergic motor neurons. Anterior is to the left in all panels. Scale bar, 8μm. (B) Representative fluorescent images of Pcasy-1c::CASY-1C::mCherry translational reporter showing co-localization with the GABAergic nuIs376 [Punc-25::SNB-1::GFP] pre-synaptic markers suggesting the presence of CASY-1C in the GABAergic NMJ pre-synaptic termini. Scale bar, 10μm. (C) Representative fluorescent images of Pcasy-1c::CASY-1C::mCherry translational reporter showing co-localization with the cholinergic nuIs152 [Punc-2129::SNB-1::GFP] pre-synaptic markers suggesting the presence of CASY-1C in the cholinergic synapses. Scale bar, 10μm.