The C-terminal of CASY-1/Calsyntenin regulates GABAergic synaptic transmission at the Caenorhabditis elegans neuromuscular junction
Fig 2
Motor neuron development is normal in casy-1 mutants.
(A) Representative fluorescent images of WT and casy-1 mutant animals expressing GFP in all GABAergic neurons (juIs76 [Punc-25:: GFP]). The number of cell bodies and axonal commissures were largely normal in casy-1 mutant animals (n-25) analyzed. Scale bar, 8μm. (B) CASY-1 functions in the mature nervous system to regulate synaptic transmission at the NMJ. Expression of CASY-1A isoform just three hours before the assay using a heat- shock promoter (hsp16.2) completely rescues the Aldicarb hypersensitivity phenotype seen in casy-1 mutant animals. The number of assays (~20 C. elegans/assay) is indicated for each genotype. (C) The C-terminal of the CASY-1A isoform is required to regulate synaptic transmission at the NMJ. Transgenic lines expressing either the CASY-1A N-terminal (ΔC) or C-terminal (ΔN) alone expressed under ins-1 promoter suggest that the CASY-1A C- terminal is sufficient to rescue the Aldicarb hypersensitivity in casy-1 mutants. Assays were done 3 times as indicated in Figures (~20 C. elegans/ assay). Data are represented as mean ± S.E.M. (***p<0.0001 using one-way ANOVA and Bonferroni's Multiple Comparison Test). “ns” indicates not significant in all Figures.