Morphogenetic defects underlie Superior Coloboma, a newly identified closure disorder of the dorsal eye
Fig 7
Developmental functions of the superior ocular sulcus.
(A) The prominent and persistent SOS (red asterisk) present in gdf6a+/- embryos aligns with the boundary between the nasal marker foxg1a and temporal marker foxd1. Note that nasal-temporal patterning is unchanged in the gdf6a heterozygotes (bottom row) compared to the wildtype embryos (top row). (B) SEM photographs showing the dorsal radial vessel (DRV) extending into the SOS (top row). DIC images of DRV (blue arrowheads) within a wide SOS (red arrows) (bottom row). Right panels are magnified views of boxed regions. (C-D) Surface projections (C) and single optical slices (D) from confocal images of Tg(rx3:GFP; kdrl:mCherry) embryos show the DRV (magenta) extending through the SOS (optic cup and lens are cyan). Scale bars are 50 μm unless otherwise noted.