The Mediator co-activator complex regulates Ty1 retromobility by controlling the balance between Ty1i and Ty1 promoters
Fig 6
Mediator occupancy at the Ty1 and Ty1i proximal promoters.
Occupancy of Med17-myc and Med15-myc from the Mediator head and tail modules, respectively, was determined by ChIP-seq and summed over all Ty1 elements in kin28-AA yeast that were otherwise wild type (WT), med20Δ, or carried the triple deletion med2Δ med3Δ med15Δ. Ty1 elements begin at 0 kb on the x-axis, and the Ty1 TSS at +238 and Ty1i TSS at +1000 are marked by green bars on the x-axis. ChIP-seq signals were normalized to an untagged kin28-AA control (S3 Fig). Reads deriving from the Ty1 TSS, and therefore in the LTR, are unavoidably also assigned to the 3’ LTR, leading to the observed signal in that region.