A molecular basis behind heterophylly in an amphibious plant, Ranunculus trichophyllus
Fig 5
Expressions of leaf polarity genes, KANs and HD-ZIPIIIs are dependent on the environment.
(A and B) Gene expression analyses of KAN genes (A), and HD-ZIPIII genes in terrestrial and aquatic leaves. (C and D) Transcript levels of leaf polarity genes after chemical treatments. The data are presented as means ± SD from three biological and two technical replicates. ACC, an ethylene precursor, was treated as ethylene. *P < 0.05; **P < 0.01 (unpaired Student’s t-test) (E) Model of heterophyllic developments regulated by ABA and ET activating leaf polarity genes, KANs and HD-ZIPIIIs, respectively. K; KANADIs, H; HD-ZIPIIIs. (F-M) Whole mount in situ hybridization for RtKANa (F-I) and RtHD-ZIPIIIa (J-M). Ab, abaxial side; Ad, adaxial side.