The non-classical nuclear import carrier Transportin 1 modulates circadian rhythms through its effect on PER1 nuclear localization
Fig 5
Oxidative stress leads to increased PER1-TNPO1 binding.
Immunoprecipitation (IP) studies as described in Fig 3B were performed either under normal (filled bars) or oxidative stress (empty bars) conditions (200 μM H2O2). Shown are bioluminescence intensities upon MYC- or V5-CoIPs (red) normalized counts from IgG control IPs (black). Given are means ± SD, n = 3 independent IPs. One-way ANOVA revealed significant difference between columns (p < 0.001) except for the IP condition PER2-V5 TNPO1-LUC; Bonferroni post-test: n.s. = non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001. Additional experiments gave similar results. For the IPs without H2O2 data from Fig 3B are re-plotted for comparison.