Rewired RNAi-mediated genome surveillance in house dust mites
Fig 5
Characteristics of small RNA-mediated TE control in dust mites.
A. siRNA producing TE-control master loci (ML). Read density of all mapping events to the positive strand in red, negative strand in blue. Density of uniquely mapping reads in yellow for positive strand and green for negative strand. B. Catalog of TE homology sequences in master loci. Multiple sequence alignment of TEs against master loci to show homologous sequences. C. Northern blots against ML-associated siRNAs (ML-A siRNA) after eliciting RNAi against dust mite Dicers. D. Northern blots against ML-A siRNAs after β-elimination test. E. Accumulation of ML-A siRNAs following incubation with the monophosphate specific terminator ribonuclease (term) and Calf intestinal phosphatase (CIP). Relative accumulation of ML-A siRNAs was determined by densitometry and normalization to U6 signal. Experiments were performed at least three times, representative results shown.