Filling gaps in bacterial amino acid biosynthesis pathways with high-throughput genetics
Fig 5
Serine biosynthesis in Burkholderia phytofirmans.
(A) A heatmap of gene fitness of the putative phosphoglycerate dehydrogenase (“FAD-linked oxidase”) and another serine biosynthesis gene (the aminotransferase serC). Our standard minimal media for this organism contains glucose and ammonia, and CAS is short for casamino acids, which contains both L-serine and L-histidine. (B) A comparison of gene fitness during growth in minimal media (x axis) or in media that was supplemented with 1 mM L-serine (y axis). The lines show x = 0, y = 0, or x = y. We highlight the genes from part (A) as well as the catabolic serine dehydratase. The other point near serC and the dehydrogenase is a putative cell wall synthesis gene (BPHYT_RS14855, ADP-L-glycero-D-manno-heptose-6-epimerase). Each point shows the average of two replicate experiments.