Sem1 links proteasome stability and specificity to multicellular development
Fig 5
Proteins co-identified with Sem1 and 19S RP in the presence or absence of Sem1.
(A) 34 proteins associated with 19S RP proteins fused to GFP in strains carrying Sem1. Proteins were identified in three biological replicates plotted as heat map representing LFQ intensities. HypoP1 and HypoP2 are conserved hypothetical proteins, with orthologs only among Aspergillus-related species. HypoP1 (AN2234) has no known conserved domain, whereas HypoP2 (AN4931) contains an Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase domain (accession number COGO365). Lower panel: schematic representation of 30 proteins plotted in a heat map. Nas6, Hsp90, Uch24 and HypoP1 are not represented. (B) 33 proteins associated with 19S RP strains lacking sem1 (marked withΔ) identified in two biological replicates plotted as heat map representing LFQ intensities. Lower panel: representation of 20 proteins identified with all RP. 9 proteins are not represented including Nas6, Hsp90, Mpp and 6 mitochondria related proteins. Proteins identified with only one tagged RP protein and/or with MSMS counts ≥5 were not represented (total 4). Heat maps representing MS/MS counts were plotted in S5 Fig. Overexpressing GFP strain served as control. In the area of the heat map were LFQ intensities and MS/MS counts are low, proteins were considered identified only when both criteria were fulfilled: LFQ>22, MS/MS counts >4. (see also S4 and S5 Figs and S1 and S2 Tables).