Conditional knockout mice for the distal appendage protein CEP164 reveal its essential roles in airway multiciliated cell differentiation
Fig 2
Ablation of CEP164 in the FOXJ1-positive tissues results in loss of airway and ependymal multicilia and hydrocephalus.
(A) Hematoxylin and eosin (H&E)-stained tracheal and sinus sections from control CEP164fl/fl and FOXJ1-Cre;CEP164fl/fl adult mice. Scale bar, 20 μm. (B) Shown are lateral views of postnatal day 18 (P18) mice (left panels), coronal sections of adult brains (middle panels), and IF staining for A-tub (green) and β-catenin (red) in whole mounts of the adult subventricular zone (SVZ) (right panels). Asterisks denote enlarged lateral ventricular spaces indicative of hydrocephalus. Scale bar, 25 μm.