Use of a microfluidic platform to uncover basic features of energy and environmental stress responses in individual cells of Bacillus subtilis
Fig 5
Responses of RsbRA- and RsbRC-only cells to different ethanol concentrations.
A. Kymographs of individual cell lineages of strains containing RsbRA or RsbRC as the only RsbR in the cell (MTC1761 or 1765, respectively). Ethanol was added at the indicated concentrations at t = 0 (dashed lines). B. Single-cell intensity traces of a stress-responsive PrsbV-mNeonGreen reporter before and after (dashed line) the addition of ethanol at the indicated concentrations with the indicated strains. C. Overlaid average intensity traces of the cell populations in B showing different ethanol concentrations. The traces were manually curated to eliminate cell lineages with cell-death events or other anomalies; comparisons of mean traces for curated and uncurated populations are shown in S5 Fig. D. Coefficient-of-variance plot comparing the RsbRA-only strain at 4% ethanol with the RsbRC-only strain at 2% ethanol. The cell populations in B were used for the computation.