Combinatorial Control of Light Induced Chromatin Remodeling and Gene Activation in Neurospora
Fig 2
Cistrome analysis of WCC and SUB1.
A. Heat-map showing the light-induced WCC occupancy at 92 binding sites identified by both, MNase-WC2 ChIP-seq and TAP-WC2 ChIP-seq. 5 kb region covering the binding sites are shown. Left panel: WCC binding in the dark. Right panel: WCC binding 30 min after light-exposure. B. Occurrence of tandem GATC motifs with the indicated spacing at WCC binding sites. 300 bp DNA regions covering the peaks of 92 highly confident WCC binding sites were analyzed. The dashed line corresponds to the occurrence of tandem GATC motifs in a set of randomly chosen 300 bp regions. C. Potential light response elements (LREs) at WCC binding sites contain multiple GATC motifs. GATC motifs in WCC binding sites of the indicated genes are shown. frqas: frq antisense [19]. vvdprox and vvddis: proximal and distal WCC binding sites in vvd promoter (S2 Table). D. Distribution of tandem GATC motifs with < 30 bp spacing at WCC binding sites. The grey area represents the sequence coverage of the WCC ChIP (MNase-WC2 ChIP, 30 min) at the highly confident 92 WCC binding sites. The red line shows the occurrence of tandem GATC motifs. E. Heat-map showing the SUB1 occupancy at binding sites. Left panel: SUB1 binding in the dark. Right panel: SUB1 binding 30 min after light-exposure. F. SUB1 binding motifs identified by MEME are shown. The major sequence motif shown in the upper panel is found in 171 sites. The GTA-rich motifs shown in the lower left and right panels are present in 82 and 63 sites, respectively. G. Distribution of the major SUB1 binding motif (a/cGAT-x6-a/cTGc/t) at SUB1 binding sites. The grey area represents the sequence coverage of the SUB1 ChIP (SUB1 30 min) at 617 SUB1 binding sites. The red line shows the occurrence of the SUB1 binding motif.