24-Hour Rhythms of DNA Methylation and Their Relation with Rhythms of RNA Expression in the Human Dorsolateral Prefrontal Cortex
Figure 7
The effects of sex, age, Alzheimer's disease, and 24-hour activity rhythms on the amplitude of rhythms of methylation.
Amplitudes expressed as percentage of mean methylation at each site for 21,282 high amplitude DNA methylation sites. Horizontal lines indicate medians. Boxes indicate interquartile ranges. P-values calculated using the Wilcoxon rank-sum test. (A) Female vs. Male Sex. (B) Age above vs. below median (88.4 years). (C) Present vs. absent Alzheimer's Disease based on NIA-Reagan Criteria (intermediate/high = present; low/no = absent). (D) High vs. low actigraphic rhythmicity based on 24-hour activity rhythms determined ante-mortem in a subset of 134 participants by actigraphy and quantified using the interdaily stability metric as described in the text. Greater age, presence of AD by NIA-Reagan criteria, female sex, and lower ante-mortem actigraphic rhythmicity were associated with a lower median amplitude of cycling.