Bipartite Recognition of DNA by TCF/Pangolin Is Remarkably Flexible and Contributes to Transcriptional Responsiveness and Tissue Specificity of Wingless Signaling
Figure 9
Identification of a W-CRM at 3R:24.4M that is active in the ring gland.
(A) Cartoon of the W-CRM in relation to the fkh gene, with the location of HMG (red arrows) and Helper (blue arrows) sites indicated, along with their spacing. Asterisks indicate the high scoring FF1 motif identified in the initial computational search. (B) Confocal images of a 3rd instar larval ring gland from a 3R:24.4M fly immunostained for lacZ and Wg. The large lateral cells of the prothoracic gland (PG) display strong nuclear LacZ staining. Wg protein can be seen at the cortical surface of these cells. (C, C′) Confocal images of ring glands from phmGal4 (C) or PhmGal4>TCFDN (C′) ring glands containing the 3R:24.4M transgene immunostained for lacZ and DAPI. TCFDN expression results in a dramatic reduction in lacZ expression in The PG. (D) Normalized pixel intensity of the LacZ reporter was calculated for 120 nuclei representing each condition, and the data were summarized using a Tukey box plot, with outliers represented by circles.