Retinoic Acid-Related Orphan Receptor γ (RORγ): A Novel Participant in the Diurnal Regulation of Hepatic Gluconeogenesis and Insulin Sensitivity
Figure 5
RORγ regulates the circadian expression of genes involved in gluconeogenesis and glycolysis pathways.
(A) Circadian expression pattern of G6pase, Pepck, Glut2, Pklr, Gck, Gckr, Gys2, Pparδ, and Dlat in liver of WT(ND) and RORγ−/−(ND) mice (n = 4). RNA was isolated every 4 h over a period of 24 h. (B) Pklr protein levels at ZT4 and ZT16 in whole liver lysates prepared from WT and RORγ−/− mice fed either a ND or HFD (n = 2–3). Pklr was examined by Western blot analysis. (C) Differential expression of several metabolic genes in liver of WT(HFD) and RORγ−/−(HFD) mice collected at ZT0 and ZT12 (n = 5). (D) Differential expression of Pcx and Klf15 in WT and RORγ−/− livers collected at ZT12. (E) Adenovirus mediated over-expressing of RORγ in RORγ−/− liver enhanced the expression of several glucose metabolic genes. (F) G6pase, Pepck, Gck, Glut2, and Gys2 expression in primary hepatocytes isolated from RORγ−/− mice (n = 3) infected with either empty or RORγ lentivirus. Data represent mean ±SD, * P<0.05, ** P<0.01, *** P<0.001 by ANOVA.