Budding Yeast ATM/ATR Control Meiotic Double-Strand Break (DSB) Levels by Down-Regulating Rec114, an Essential Component of the DSB-machinery
Figure 5
Effect of Rec114 phosphorylation on its synapsis dependent removal.
A. Temporal and spatial dynamics of Rec114 and Zip1 localization are assessed cytologically using antibodies against each protein. Presented are representative images of cells in leptotene/zygotene (i); zygotene/pachytene (ii); and pachytene (iii). The classification was based on the extent of Zip1-polymerization. White arrowheads: examples of the mutual exclusiveness of Rec114 and Zip1 signals. Scale bar: 5 µm. B. The fraction of REC114 ndt80Δ cells with Rec114 foci (black lines) or Zip1-linear stretches (orange lines). Grey columns; the average number of Rec114 foci per cell. C. (i) Fraction of Rec114-foci co-localizing with either Zip1-foci (yellow) or Zip1-lines (green). For each time point, ∼500 Rec114-foci collected from ∼ REC114 ndt80Δ nuclei were analyzed. (ii) Fraction of Zip1-lines co-localizing with Rec114-foci in the same ∼50 REC114 ndt80Δ nuclei per time point analyzed in panel (i). D. The average number of Rec114 foci (i), fraction of cells containing Rec114 foci (ii), and fraction of cells containing Zip1-linear stretches (iii) in REC114 ndt80Δ (green), rec114-8A ndt80Δ (red) or rec114-8D ndt80Δ (blue) cells.