Patterning of Leaf Vein Networks by Convergent Auxin Transport Pathways
Figure 5
Necessity of PIN5, PIN6, and PIN8 for vein network formation.
(A) Vein network complexity as mean ± SE number of vein branching points per first-leaf area unit in mm2 [28]. Difference between pin6;8 and all other genotypes was significant at P<0.01 (**) by one-way ANOVA and Tukey's test. Sample population sizes: WT, 30; pin5, 30; pin6, 30; pin8, 27; pin5;6, 28; pin5;8, 28; pin6;8, 28; pin5;6;8, 28. (B–N) Top right: genotype, markers and leaf age in days after germination (DAG). Bottom left: reproducibility index. (B–D) Dark-field illumination of cleared mature first leaves. (E–N) Confocal laser scanning microscopy; first leaves. LUT (in Figure 2K) visualizes expression levels. (E–G) DR5rev::YFPnuc expression, 4 DAG. Dotted line: leaf outline. (H–N) PIN1::PIN1:YFP expression. (O) 4-DAG first leaf illustrating positions of close-ups in (E–G) and (H–N). Bars: (B–D) 1.5 mm; (E–G,L–N) 50 µm; (H–K) 25 µm.