Regulation of Drosophila Metamorphosis by Xenobiotic Response Regulators
Figure 2
Regulation of early puff gene transcription by CncC and dKeap1.
(A). Effects of CncC depletion in the salivary glands on the transcription of ecdysone-regulated genes and xenobiotic response genes. The images show wild type polytene chromosomes stained using anti-CncC antibodies. The levels of the transcripts indicated below the bar graphs were measured in the salivary glands of early wandering 3rd instar larvae. Transcript levels were compared in control larvae [71B-GAL4 (71B>), Sgs3-GAL4 (Sgs3>); open bars] and in larvae that expressed the shRNA targeting CncC under the control of the 71B-GAL4 (71B>cncC-RNAi; solid bars) or the Sgs3-GAL4 (Sgs3>cncC-RNAi; striped bars) driver, as well as in larvae that expressed a different shRNA targeting all Cnc isoforms under the control of the 71B-GAL4 driver (71B>cnc-RNAi; dotted bars). Larvae with different drivers and shRNAs were analyzed in separate experiments under different conditions (see methods). To facilitate comparison, the transcript levels in the lower graph were normalized by the levels of the transcripts in the control larvae. (B). Effects of dKeap1 depletion in the salivary glands on the transcription of ecdysone-regulated genes and xenobiotic response genes. The levels of the transcripts indicated below the bar graphs were measured in the salivary glands of early wandering 3rd instar larvae that expressed the shRNA targeting dKeap1 under the control of the Sgs3-GAL4 driver as described for the lower graph in panel A. The data in panels A and B represent the means and standard deviations from three or four separate experiments each (*, p<0.05). The corresponding loci are indicated in parentheses below the lower graph.