Single-Stranded Annealing Induced by Re-Initiation of Replication Origins Provides a Novel and Efficient Mechanism for Generating Copy Number Expansion via Non-Allelic Homologous Recombination
Figure 5
Flanking repetitive elements must be re-replicated in order for RRIGA to occur.
A) Schematic showing relocation of the RA3 and UR elements (3′ and 5′ portions of URA3, respectively) used in the URA3 RRIGA selection assay to change their position relative to the re-initiating origin (ARS317, light blue line) and the distribution of re-replication forks (as inferred from the re-replication profile). B) Induced amplification frequencies (mean ± SEM, n = 3) for strains with the indicated amplicon boundaries as defined by the position of the URA3 fragments. Induced frequency is frequency after 3 hr re-replication minus frequency after 0 hr re-replication.