Base Damage within Single-Strand DNA Underlies In Vivo Hypermutability Induced by a Ubiquitous Environmental Agent
Figure 5
The mutation spectra of triple loss-of-function isolates induced by 1% sodium bisulfite.
(A) The reporter gene region of 30 CanR Ura− Ade− isolates obtained from bisulfite treatment of UNG1 cells was sequenced. (B) The reporter gene region of 23 CanR Ura− Ade− isolates obtained from bisulfite treatment of ung1Δ cells was sequenced. In both cases, >75% of mutations were C to T transitions, confirming that bisulfite-induced mutagenesis is independent of UNG1 genotype. Table S4 lists bisulfite-induced mutations, while Table S6 lists mutations found in buffer only controls.