Specification of Drosophila Corpora Cardiaca Neuroendocrine Cells from Mesoderm Is Regulated by Notch Signaling
Figure 6
Tinman expression in head mesoderm is regulated by Notch signaling.
(A) Glass expression in stage 12 wild type embryonic head region. CC precursors are located in the dorsal midline (red arrowheads). Black arrowheads indicate Glass+ larval eye precursors while white arrowheads indicate Glass+ brain primordia. (B) In da mutant at stage 12, both CC (red arrowheads) and eye precursors (black arrowheads) are missing, but Glass expression in the brain primordia (white arrowheads) is maintained. (C) In twi mutant at stage 12, CC precursors are missing in the dorsal midline (red arrowheads) while brain (white arrowheads) and eye precursors (black arrowheads) are intact. (D) In tin mutant at stage 12, CC precursors are missing in the dorsal midline (red arrowheads). (E) In stage12 Df(1)BSC530 mutant in which proneural genes are removed, CC precursors (red arrowheads) are intact. (F, G) Dorsal view of stage 11 wild type embryonic head shows Glass+ CC precursors (arrowhead and green in insert of F) co-localized with Twist+ cells (red). (H, I) Dorsal view of stage 11 Notch mutant shows multiple Glass+ CC precursors (outlined and green in insert) co-localized with Twist+ cells (red). (J) Twi expression in head mesoderm is maintained in stage 11 embryo when Notch signaling is activated in mesoderm by twi.2×PE-GAL4; UAS-Nintra. (K, L) Dorsal view of stage 11 wild type embryonic head shows Glass+ CC precursors (arrowhead and green in K) co-localized with Tinman+ cells (red). (M, N) Dorsal view of stage 11 Notch mutant shows multiple Glass+ CC precursors (outlined and green in insert) co-localized with Tinman+ cells (red). (O) Both Glass and Tinman expression in head mesoderm is abolished at stage 11 embryo when Notch signaling is activated in mesoderm by twi.2×PE-GAL4; UAS-Nintra. All embryo images are dorsal views with anterior to the left.