Comparative and Functional Genomics of Rhodococcus opacus PD630 for Biofuels Development
Figure 4
Identification and purification of odd-carbon straight-chain fatty acids generated by Rhodococcus.
a and b) GC-FID analysis of FAMEs synthesized during fermentation. Freeze-dried whole cells fermented on glucose of R. opacus PD630 (a) and R. jostii RHA1 (b). c and d) GC-FID analysis of FAMEs derived from TLC-purified TAGs of R. opacus PD630 (c) and R. jostii RHA1 (d) grown on glucose e) odd lipids C15∶0, C17∶0, and C17∶1 increase during fermentation of both R. opacus PD630 and R. jostii RHA1. f) Rhodococcus grown on propionate generates mostly C15∶0, C17∶0, and C17∶1 fatty acids that were used for purification and confirmation by mass spectrometry and structural 1H-NMR. These analyses demonstrated that the purified Rhodococcus odd-carbon fatty acids were straight-chain.