A Role for Phosphatidic Acid in the Formation of “Supersized” Lipid Droplets
Figure 5
SLD formation in yeast cells deficient in PA phosphatase activity.
A&B) Formation of SLDs in pah1Δ, but not in dga1Δ or dga1Δ lro1Δ strains. Bar, 5 µm. C) Relative cellular TAG amounts quantified by thin layer chromatography and densitometry. D) Addition of 1 mM membrane-permeable DAG analog (1,2-dioctanoyl-sn-glycerol) and 1 mM oleate, but not oleate alone, significantly elevated the percentage of pah1Δ cells accumulating supersized LDs. Cells with supersized LDs were counted and the percentages were presented as mean±SD.