Bacteriophage Crosstalk: Coordination of Prophage Induction by Trans-Acting Antirepressors
Figure 5
Monitoring Gifsy phage repressors and antirepressors under inducing conditions.
Strains harboring 3xFLAG-tagged versions of both repressor and antirepressor genes in prophage Gifsy-1 (A; strain MA8729), Gifsy-2 (B; strain MA8728) or of the antirepressor gene in Fels-1 (C; MA8605) were exposed to Mitomycin C (1 µg mL−1) for 30 or 60 min. Chloramphenicol (10 µg mL−1) was added to samples subjected to the 60 min treatment and incubation continued for additional 30 min or 60 min. Bacteria were harvested and processed for immunodetection of epitope-tagged proteins as described [47].