Reduction of Protein Translation and Activation of Autophagy Protect against PINK1 Pathogenesis in Drosophila melanogaster
Figure 4
RpS6 or RpS9 RNAi blocks the enhancing effects of S6K-TE in PINK1 RNAi background.
Overexpression of RpS6 or RpS9 RNAi transgenes in PINK1 RNAi or PINK1 RNAi/UAS-S6K-TE flies efficiently rescued the abnormal wing posture (A), thoracic indentation (B), and energy depletion (D) phenotypes in 1-day-old flies and partially suppressed these phenotypes in 14-day-old flies. Data are presented as mean ± s.e.m. Statistical significance was determined by Student's t test (*P<0.001). (C) RpS6 or RpS9 RNAi blocked increased mitochondrial aggregation in PINK1 RNAi, UAS-S6K-TE flies. The scale bar represents 5 µm. (E) Western blot analysis comparing the levels of dS6K and phosphorylated S6K (T398) in wild type, Mhc-Gal4>PINK1 RNAi and PINK1B9 mutant flies. The phosphorylation of S6K was significantly decreased in PINK1 RNAi or mutant flies.