The Five AhMTP1 Zinc Transporters Undergo Different Evolutionary Fates towards Adaptive Evolution to Zinc Tolerance in Arabidopsis halleri
Figure 6
Functional complementation of the zinc-hypersensitivity of the zrc1 cot1 yeast mutant by AhMTP1s and AtMTP1.
Wild-type BY4741 (WT) and mutant zrc1 cot1 yeast strains harboured the empty pFL38H (EV1) that brought histidine autotrophy and either empty pYX212 vector (EV2) or pYX212 bearing one of MTP1s. Serial dilutions were spotted on modified selective LSP medium supplemented with different concentrations of ZnSO4 as indicated above the panels. Each spot was made with 10 µl of a yeast culture diluted at the OD600nm mentioned below the drops. Pictures were taken after 2 days for control and 4 days for other treatments.