VEZF1 Elements Mediate Protection from DNA Methylation
Figure 4
Nuclear proteins specifically interact with the dG-dC strings at HS4 footprints I, III, and V.
(A–C) Gel mobility shift analysis of interactions between chicken adult erythrocyte nuclear extract and 32P-labelled FI (A), FIII (B), and FV (C) oligonucleotide duplexes. Unlabelled competitor duplexes (indicated above each lane) were added at 50 fold molar excess. Arrows indicate footprint sequence-specific complexes. Asterisks indicate a non-specific FI complex in (A) and an FV complex that was not pursued further due to inconsistent abundance in (C). (D) Sequences present in competitor oligonucleotides. Mutations shown in bold lower case. Footprinted bases are indicated by shading. Bases that are essential for maximal binding are underlined; mutations of other bases had no effect on binding (A–C, data not shown).