Characterization of METTL3/14-mediated m6A modification in human transcriptome using Nanopore direct RNA sequencing

Emily Kurtyan; Andrew J. Stein; Kelly J. Abdalla; Zhangerjiao Yuan; Miten Jain; Fadia Ibrahim

doi:10.1371/journal.pgen.1012278

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Open Access

Peer-reviewed

Research Article

Characterization of METTL3/14-mediated m⁶A modification in human transcriptome using Nanopore direct RNA sequencing

Emily Kurtyan ,

Contributed equally to this work with: Emily Kurtyan, Andrew J. Stein

Roles Methodology, Validation, Visualization, Writing – review & editing

Affiliation Department of Biochemistry and Molecular Biology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America

https://orcid.org/0009-0001-0332-0865

⨯
Andrew J. Stein ,

Contributed equally to this work with: Emily Kurtyan, Andrew J. Stein

Roles Formal analysis, Methodology, Software, Visualization, Writing – review & editing

Affiliation Department of Bioengineering, Northeastern University, Boston, Massachusetts, United States of America

https://orcid.org/0009-0001-6525-6512

⨯
Kelly J. Abdalla,

Roles Formal analysis, Methodology, Software, Visualization, Writing – review & editing

Affiliation Department of Biochemistry and Molecular Biology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America
⨯
Zhangerjiao Yuan,

Roles Methodology, Visualization, Writing – review & editing

Affiliation Department of Biochemistry and Molecular Biology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America
⨯
Miten Jain,

Roles Funding acquisition, Supervision, Validation, Writing – review & editing

Affiliations Department of Bioengineering, Northeastern University, Boston, Massachusetts, United States of America, Department of Physics, Northeastern University, Boston, Massachusetts, United States of America, Khoury College of Computer Sciences, Northeastern University, Boston, Massachusetts, United States of America
⨯
Fadia Ibrahim

Roles Conceptualization, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: fadia.ibrahim@jefferson.edu

Affiliation Department of Biochemistry and Molecular Biology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America

https://orcid.org/0000-0002-4344-765X

⨯

Characterization of METTL3/14-mediated m⁶A modification in human transcriptome using Nanopore direct RNA sequencing

Emily Kurtyan,
Andrew J. Stein,
Kelly J. Abdalla,
Zhangerjiao Yuan,
Miten Jain,
Fadia Ibrahim

Published: September 1, 2026
https://doi.org/10.1371/journal.pgen.1012278

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RNA sequencing
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Small interfering RNA
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Gene expression
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DNA methylation
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Sequence motif analysis
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