A point mutation in the FAT domain constitutively increases the kinase activity of Rad3^ATR and bypasses the requirement for 9-1–1 phosphorylation to activate the DNA replication checkpoint

• Kamal Dev,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – review & editing

  Affiliation Department of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, Ohio, United States of America

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• S. Dean Rider Jr.,

  Roles Formal analysis

  Affiliation Department of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, Ohio, United States of America

  https://orcid.org/0000-0002-5792-3768

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• Balveer Singh,

  Roles Investigation

  Affiliation Department of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, Ohio, United States of America

  https://orcid.org/0009-0009-0558-9137

  ⨯
• Abhinav Saini,

  Roles Investigation

  Affiliation Department of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, Ohio, United States of America

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• Yong-jie Xu

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Supervision, Validation, Writing – original draft, Writing – review & editing

  * E-mail: yong-jie.xu@wright.edu

  Affiliation Department of Pharmacology and Toxicology, Boonshoft School of Medicine, Wright State University, Dayton, Ohio, United States of America

  https://orcid.org/0000-0002-5091-5247

  ⨯