Serine/threonine protein kinase phosphorylation of DosR alters target gene transcription mechanics and regulates Mycobacterium tuberculosis response to nitric oxide stress

Natalie R. Sontag; Ana Ruiz Manzano; Alwyn M. V. Ecker; Eric A. Galburt; Shumin Tan

doi:10.1371/journal.pgen.1012043

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Peer-reviewed

Research Article

Serine/threonine protein kinase phosphorylation of DosR alters target gene transcription mechanics and regulates Mycobacterium tuberculosis response to nitric oxide stress

Natalie R. Sontag,

Roles Conceptualization, Investigation, Writing – original draft, Writing – review & editing

Affiliations Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, United States of America, Graduate Program in Molecular Microbiology, Graduate School of Biomedical Sciences, Tufts University, Boston, Massachusetts, United States of America

https://orcid.org/0000-0003-3599-9988

⨯
Ana Ruiz Manzano,

Roles Investigation, Writing – review & editing

Affiliation Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri, United States of America

https://orcid.org/0000-0001-9135-1436

⨯
Alwyn M. V. Ecker,

Roles Investigation

Affiliation Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, United States of America

https://orcid.org/0000-0002-1619-3885

⨯
Eric A. Galburt ,

Roles Funding acquisition, Supervision, Writing – review & editing

* E-mail: Shumin.Tan@tufts.edu (ST); egalburt@wustl.edu (EAG)

Affiliation Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri, United States of America

https://orcid.org/0000-0003-4314-3215

⨯
Shumin Tan

Roles Conceptualization, Funding acquisition, Investigation, Supervision, Writing – original draft, Writing – review & editing

* E-mail: Shumin.Tan@tufts.edu (ST); egalburt@wustl.edu (EAG)

Affiliations Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, United States of America, Graduate Program in Molecular Microbiology, Graduate School of Biomedical Sciences, Tufts University, Boston, Massachusetts, United States of America

https://orcid.org/0000-0003-1093-2576

⨯

Serine/threonine protein kinase phosphorylation of DosR alters target gene transcription mechanics and regulates Mycobacterium tuberculosis response to nitric oxide stress

Natalie R. Sontag,
Ana Ruiz Manzano,
Alwyn M. V. Ecker,
Eric A. Galburt,
Shumin Tan

Published: February 12, 2026
https://doi.org/10.1371/journal.pgen.1012043

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Mycobacterium tuberculosis
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Phosphorylation
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Electrophoretic mobility shift assay
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DNA transcription
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Transcriptional control
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Gene regulation
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Regulons
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Nitric oxide
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