Refined CRISPR/Cas9 genome editing in the pea aphid uncovers the essential roles of Laccase2 in overwintering egg adaptation

• Shuji Shigenobu ,

  Contributed equally to this work with: Shuji Shigenobu, Shinichi Yoda

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: shige@nibb.ac.jp

  Affiliations Laboratory of Evolutionary Genomics, Trans-Scale Biology Center, National Institute for Basic Biology, Okazaki, Japan, Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Japan, Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki, Japan

  https://orcid.org/0000-0003-4640-2323

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• Shinichi Yoda ,

  Contributed equally to this work with: Shuji Shigenobu, Shinichi Yoda

  Roles Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Laboratory of Evolutionary Genomics, Trans-Scale Biology Center, National Institute for Basic Biology, Okazaki, Japan

  https://orcid.org/0000-0003-4720-736X

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• Sonoko Ohsawa,

  Roles Formal analysis, Investigation, Methodology, Writing – original draft

  Affiliation Laboratory of Evolutionary Genomics, Trans-Scale Biology Center, National Institute for Basic Biology, Okazaki, Japan

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• Miyuzu Suzuki

  Roles Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Laboratory of Evolutionary Genomics, Trans-Scale Biology Center, National Institute for Basic Biology, Okazaki, Japan

  https://orcid.org/0000-0003-4269-7523

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