Single-step generation of homozygous knockout/knock-in individuals in an extremotolerant parthenogenetic tardigrade using DIPA-CRISPR

• Koyuki Kondo,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – original draft, Writing – review & editing

  Affiliations Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan, Department of Life Science, Faculty of Advanced Engineering, Chiba Institute of Technology, Tsudanuma, Narashino, Chiba, Japan

  https://orcid.org/0000-0002-5776-3370

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• Akihiro Tanaka,

  Roles Funding acquisition, Methodology, Resources, Writing – review & editing

  Current address: Department of Chromosome Science, National Institute of Genetics, Yata, Mishima, Shizuoka, Japan

  Affiliation Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan

  https://orcid.org/0000-0002-1572-2888

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• Takekazu Kunieda

  Roles Conceptualization, Data curation, Funding acquisition, Project administration, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: kunieda@bs.s.u-tokyo.ac.jp

  Affiliation Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan

  https://orcid.org/0000-0002-6256-1335

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