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Open Access
Peer-reviewed
Research Article
Long-read sequencing for fast and robust identification of correct genome-edited alleles: PCR-based and Cas9 capture methods
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Christopher V. McCabe ,
Roles Investigation, Methodology, Visualization
¶‡ These authors are joint senior authors on this work.
Affiliation The Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
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Peter D. Price ,
Roles Data curation, Formal analysis, Investigation, Methodology, Software, Writing – original draft
¶‡ These authors are joint senior authors on this work.
Affiliation The Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
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Gemma F. Codner ,
Roles Conceptualization, Data curation, Formal analysis, Methodology, Resources, Supervision, Writing – original draft, Writing – review & editing
¶‡ These authors are joint senior authors on this work.
Affiliation The Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
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Alasdair J. Allan,
Roles Conceptualization, Formal analysis, Investigation, Resources, Supervision
Affiliation The Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
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Adam Caulder,
Roles Investigation, Resources, Supervision
Affiliation The Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
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Skevoulla Christou,
Roles Data curation, Formal analysis, Investigation, Methodology, Software, Visualization, Writing – original draft
Affiliation The Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
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Jorik Loeffler,
Roles Investigation, Methodology, Writing – original draft
Affiliation The Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
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Matthew Mackenzie,
Roles Investigation, Methodology, Resources, Writing – review & editing
Affiliation The Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
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Elke Malzer,
Roles Investigation, Resources, Writing – review & editing
Affiliation The Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
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Joffrey Mianné,
Roles Investigation, Methodology, Resources, Writing – review & editing
Affiliation The Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
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Krystian J. Nowicki,
Roles Formal analysis, Investigation, Methodology, Software, Visualization, Writing – review & editing
Affiliation The Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
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Edward J. O’Neill,
Roles Formal analysis, Investigation, Resources, Software, Visualization
Affiliation The Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
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Fran J. Pike,
Roles Investigation, Resources, Writing – review & editing
Affiliation The Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
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Marie Hutchison,
Roles Resources, Supervision
Affiliation The Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
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Benoit Petit-Demoulière,
Roles Software
Affiliation Université de Strasbourg, CNRS, INSERM, Institut Clinique de la Souris (ICS), PHENOMIN, CELPHEDIA, Illkirch, France
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Michelle E. Stewart,
Roles Resources, Supervision
Affiliation The Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
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Hilary Gates,
Roles Funding acquisition, Project administration, Resources
Affiliations The Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom, Mammalian Genetics Unit, MRC Harwell, Oxfordshire, United Kingdom
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Sara Wells,
Roles Funding acquisition, Resources, Supervision, Writing – review & editing
Affiliation The Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
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Nicholas D. Sanderson,
Roles Formal analysis, Investigation, Methodology, Software, Supervision, Writing – review & editing
Affiliation Nuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom
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Lydia Teboul
Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Supervision, Visualization, Writing – original draft, Writing – review & editing
* E-mail: l.teboul@har.mrc.ac.uk
Affiliation The Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
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Long-read sequencing for fast and robust identification of correct genome-edited alleles: PCR-based and Cas9 capture methods
- Christopher V. McCabe,
- Peter D. Price,
- Gemma F. Codner,
- Alasdair J. Allan,
- Adam Caulder,
- Skevoulla Christou,
- Jorik Loeffler,
- Matthew Mackenzie,
- Elke Malzer,
- Joffrey Mianné
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- Published: March 8, 2024
- https://doi.org/10.1371/journal.pgen.1011187