Structural transitions upon guide RNA binding and their importance in Cas12g-mediated RNA cleavage

• Mengxi Liu ,

  Contributed equally to this work with: Mengxi Liu, Zekai Li

  Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Key Laboratory of Microbial Pathogenesis and Interventions-Fujian Province University, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China

  https://orcid.org/0000-0002-1191-3249

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• Zekai Li ,

  Contributed equally to this work with: Mengxi Liu, Zekai Li

  Roles Data curation, Formal analysis

  Affiliation Key Laboratory of Microbial Pathogenesis and Interventions-Fujian Province University, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China

  ⨯
• Jing Chen,

  Roles Investigation, Methodology

  Affiliation Key Laboratory of Microbial Pathogenesis and Interventions-Fujian Province University, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China

  ⨯
• Jinying Lin,

  Roles Investigation

  Affiliation Key Laboratory of Microbial Pathogenesis and Interventions-Fujian Province University, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China

  ⨯
• Qiuhua Lu,

  Roles Investigation

  Affiliation Key Laboratory of Microbial Pathogenesis and Interventions-Fujian Province University, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China

  https://orcid.org/0009-0004-1793-7868

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• Yangmiao Ye,

  Roles Investigation

  Affiliation Key Laboratory of Microbial Pathogenesis and Interventions-Fujian Province University, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China

  https://orcid.org/0009-0006-1134-3852

  ⨯
• Hongmin Zhang,

  Roles Resources

  Affiliation Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, China

  https://orcid.org/0000-0003-4356-3615

  ⨯
• Bo Zhang ,

  Roles Conceptualization, Data curation, Formal analysis, Methodology, Project administration, Supervision, Validation

  * E-mail: bozhang@fjnu.edu.cn (BZ); ouyangsy@fjnu.edu.cn (SO)

  Affiliation Key Laboratory of Microbial Pathogenesis and Interventions-Fujian Province University, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China

  ⨯
• Songying Ouyang

  Roles Conceptualization, Funding acquisition, Project administration, Resources, Supervision

  * E-mail: bozhang@fjnu.edu.cn (BZ); ouyangsy@fjnu.edu.cn (SO)

  Affiliation Key Laboratory of Microbial Pathogenesis and Interventions-Fujian Province University, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, China

  https://orcid.org/0000-0002-1120-1524

  ⨯