Multiplexed CRISPR/Cas9-mediated knockout of 19 Fanconi anemia pathway genes in zebrafish revealed their roles in growth, sexual development and fertility

• Ramanagouda Ramanagoudr-Bhojappa ,

  Contributed equally to this work with: Ramanagouda Ramanagoudr-Bhojappa, Blake Carrington

  Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – review & editing

  Affiliation Cancer Genomics Unit, Cancer Genetics and Comparative Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America

  ⨯
• Blake Carrington ,

  Contributed equally to this work with: Ramanagouda Ramanagoudr-Bhojappa, Blake Carrington

  Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – review & editing

  Affiliation Zebrafish Core, Translational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America

  http://orcid.org/0000-0002-1672-1887

  ⨯
• Mukundhan Ramaswami,

  Roles Methodology

  Affiliation Cancer Genomics Unit, Cancer Genetics and Comparative Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America

  http://orcid.org/0000-0002-4804-5676

  ⨯
• Kevin Bishop,

  Roles Methodology

  Affiliation Zebrafish Core, Translational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America

  http://orcid.org/0000-0003-2571-4803

  ⨯
• Gabrielle M. Robbins,

  Roles Investigation, Methodology

  Affiliation Zebrafish Core, Translational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America

  http://orcid.org/0000-0001-5487-1766

  ⨯
• MaryPat Jones,

  Roles Data curation, Formal analysis, Investigation, Methodology

  Affiliation Genomics Core, Cancer Genetics and Comparative Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America

  http://orcid.org/0000-0001-8330-1279

  ⨯
• Ursula Harper,

  Roles Methodology

  Affiliation Genomics Core, Cancer Genetics and Comparative Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America

  ⨯
• Stephen C. Frederickson,

  Roles Methodology

  Affiliation Zebrafish Core, Translational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America

  http://orcid.org/0000-0002-7668-4723

  ⨯
• Danielle C. Kimble,

  Roles Data curation, Methodology

  Affiliation Cancer Genomics Unit, Cancer Genetics and Comparative Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America

  ⨯
• Raman Sood ,

  Roles Data curation, Formal analysis, Funding acquisition, Methodology, Project administration, Supervision, Visualization, Writing – original draft, Writing – review & editing

  ¶‡ These authors also contributed equally to this work.

  Affiliation Zebrafish Core, Translational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America

  http://orcid.org/0000-0001-5565-662X

  ⨯
• Settara C. Chandrasekharappa

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Methodology, Project administration, Supervision, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: chandra@mail.nih.gov

  ¶‡ These authors also contributed equally to this work.

  Affiliations Cancer Genomics Unit, Cancer Genetics and Comparative Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America, Genomics Core, Cancer Genetics and Comparative Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America

  http://orcid.org/0000-0002-8084-0530

  ⨯