Chemical Genetics Reveals a Specific Requirement for Cdk2 Activity in the DNA Damage Response and Identifies Nbs1 as a Cdk2 Substrate in Human Cells

Lara Wohlbold; Karl A. Merrick; Saurav De; Ramon Amat; Jun Hyun Kim; Stéphane Larochelle; Jasmina J. Allen; Chao Zhang; Kevan M. Shokat; John H. J. Petrini; Robert P. Fisher

doi:10.1371/journal.pgen.1002935

Loading metrics

Open Access

Peer-reviewed

Research Article

Chemical Genetics Reveals a Specific Requirement for Cdk2 Activity in the DNA Damage Response and Identifies Nbs1 as a Cdk2 Substrate in Human Cells

Lara Wohlbold,

Affiliation Department of Structural and Chemical Biology, Mount Sinai School of Medicine, New York, New York, United States of America
⨯
Karl A. Merrick,

Affiliations Department of Structural and Chemical Biology, Mount Sinai School of Medicine, New York, New York, United States of America, Program in Biochemistry and Program in Cell and Molecular Biology, Weill Cornell Graduate School of Medical Sciences, New York, New York, United States of America
⨯
Saurav De,

Affiliation Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America
⨯
Ramon Amat,

Affiliation Department of Structural and Chemical Biology, Mount Sinai School of Medicine, New York, New York, United States of America
⨯
Jun Hyun Kim,

Affiliation Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America
⨯
Stéphane Larochelle,

Affiliation Department of Structural and Chemical Biology, Mount Sinai School of Medicine, New York, New York, United States of America
⨯
Jasmina J. Allen,

Affiliation Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California, United States of America
⨯
Chao Zhang,

Affiliation Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California, United States of America
⨯
Kevan M. Shokat,

Affiliation Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California, United States of America
⨯
John H. J. Petrini,

Affiliation Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America
⨯
Robert P. Fisher

* E-mail: robert.fisher@mssm.edu

Affiliation Department of Structural and Chemical Biology, Mount Sinai School of Medicine, New York, New York, United States of America
⨯

Chemical Genetics Reveals a Specific Requirement for Cdk2 Activity in the DNA Damage Response and Identifies Nbs1 as a Cdk2 Substrate in Human Cells

Lara Wohlbold,
Karl A. Merrick,
Saurav De,
Ramon Amat,
Jun Hyun Kim,
Stéphane Larochelle,
Jasmina J. Allen,
Chao Zhang,
Kevan M. Shokat,
John H. J. Petrini

Published: August 23, 2012
https://doi.org/10.1371/journal.pgen.1002935

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Phosphorylation
Is the Subject Area "Phosphorylation" applicable to this article?

Thanks for your feedback.
DNA damage
Is the Subject Area "DNA damage" applicable to this article?

Thanks for your feedback.
DNA repair
Is the Subject Area "DNA repair" applicable to this article?

Thanks for your feedback.
Immunoprecipitation
Is the Subject Area "Immunoprecipitation" applicable to this article?

Thanks for your feedback.
Cyclins
Is the Subject Area "Cyclins" applicable to this article?

Thanks for your feedback.
Synthesis phase
Is the Subject Area "Synthesis phase" applicable to this article?

Thanks for your feedback.
Small interfering RNA
Is the Subject Area "Small interfering RNA" applicable to this article?

Thanks for your feedback.
Chromatin
Is the Subject Area "Chromatin" applicable to this article?

Thanks for your feedback.