Probing the Functional Impact of Sequence Variation on p53-DNA Interactions Using a Novel Microsphere Assay for Protein-DNA Binding with Human Cell Extracts

Maher A. Noureddine; Daniel Menendez; Michelle R. Campbell; Omari J. Bandele; Monica M. Horvath; Xuting Wang; Gary S. Pittman; Brian N. Chorley; Michael A. Resnick; Douglas A. Bell

doi:10.1371/journal.pgen.1000462

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Open Access

Peer-reviewed

Research Article

Probing the Functional Impact of Sequence Variation on p53-DNA Interactions Using a Novel Microsphere Assay for Protein-DNA Binding with Human Cell Extracts

Maher A. Noureddine ,

Contributed equally to this work with: Maher A. Noureddine, Daniel Menendez, Michelle R. Campbell

Affiliation Environmental Genomics Group, Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, United States of America
⨯
Daniel Menendez ,

Contributed equally to this work with: Maher A. Noureddine, Daniel Menendez, Michelle R. Campbell

Affiliation Chromosome Stability Group, Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, United States of America
⨯
Michelle R. Campbell ,

Contributed equally to this work with: Maher A. Noureddine, Daniel Menendez, Michelle R. Campbell

Affiliation Environmental Genomics Group, Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, United States of America
⨯
Omari J. Bandele,

Affiliation Environmental Genomics Group, Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, United States of America
⨯
Monica M. Horvath,

Affiliation Environmental Genomics Group, Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, United States of America
⨯
Xuting Wang,

Affiliation Environmental Genomics Group, Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, United States of America
⨯
Gary S. Pittman,

Affiliation Environmental Genomics Group, Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, United States of America
⨯
Brian N. Chorley,

Affiliation Environmental Genomics Group, Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, United States of America
⨯
Michael A. Resnick,

Affiliation Chromosome Stability Group, Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, United States of America
⨯
Douglas A. Bell

* E-mail: BELL1@niehs.nih.gov

Affiliation Environmental Genomics Group, Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, United States of America
⨯

Probing the Functional Impact of Sequence Variation on p53-DNA Interactions Using a Novel Microsphere Assay for Protein-DNA Binding with Human Cell Extracts

Maher A. Noureddine,
Daniel Menendez,
Michelle R. Campbell,
Omari J. Bandele,
Monica M. Horvath,
Xuting Wang,
Gary S. Pittman,
Brian N. Chorley,
Michael A. Resnick,
Douglas A. Bell

Published: May 8, 2009
https://doi.org/10.1371/journal.pgen.1000462

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Oligonucleotides
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Cell binding assay
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Cell binding
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Single nucleotide polymorphisms
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DNA-binding proteins
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Binding analysis
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Transactivation
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Protein extraction
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