Upstream open reading frames repress the translation from the iab-8 RNA
Fig 3
Multiple uORFs in iab-8 exon1&2 can repress downstream translation.
A. Summary of the results and constructs. The different constructs tested are schematized with their names indicated on the left. Above the constructs are schematic representations of the two firsts exons of the iab-8 ncRNA with all of the potential ORFs indicated by the directional boxes, color-coded based on their reading frame relative to the beginning of the transcript. The start codons of each ORF are indicated by colored triangles using the color code established for the ORFs. The exon-1&2 region was divided into sub-fragments delineated (Fragments A-F) and labeled in the top panel of A. The (and) represent DNA fragment deletions. The coding sequence of GFP (with the ATG start codon in yellow) is indicated by a solid green rectangle for constructs that express GFP and by a grey hatched box for constructs that did not based on the results displayed in panel B. Kozak sequences are indicated by light grey boxes. B. The graph shows the relative fluorescence measured (arbitrary units, Y-axis) for each of the constructs tested (X-axis). Each dot represents a measured S2 cell. The error bars indicate the median with the interquartile range. Statistical analysis was performed using the multiple comparison Kruskal-Wallis test (non-parametric) on the PRISM software. The **** indicate p<0.0001 relative to the construct indicated with the longer bar.