Murine norovirus expresses a novel protein from an alternative reading frame on the viral subgenomic RNA, which plays a role in the regulation of the innate immune response and apoptosis. VF1 is important for efficient infection of its natural host as viruses lacking VF1 are attenuated in mice (see McFadden et al., doi:10.1371/journal.ppat.1002413). The localization of VF1 to the mitochondria is shown by confocal microscopy. The expression of VF1 N-terminally fused with GFP (green) in transfected Cos7 cells co-localizes with the mitochondrial Mitotracker stain (red), while the nucleus is shown in blue using DAPI staining.
Image Credit: Guia Carrara and Ian Goodfellow, Imperial College London
Opinions
Vaccinomics and Personalized Vaccinology: Is Science Leading Us Toward a New Path of Directed Vaccine Development and Discovery?
PLOS Pathogens: published December 29, 2011 | https://doi.org/10.1371/journal.ppat.1002344
Nutritional Immunology: A Multi-Dimensional Approach
PLOS Pathogens: published December 1, 2011 | https://doi.org/10.1371/journal.ppat.1002223
Pearls
Five Questions about Non-Mevalonate Isoprenoid Biosynthesis
PLOS Pathogens: published December 22, 2011 | https://doi.org/10.1371/journal.ppat.1002323
Comparative Pathobiology of Fungal Pathogens of Plants and Animals
PLOS Pathogens: published December 15, 2011 | https://doi.org/10.1371/journal.ppat.1002324
Review
The Meaning of Death: Evolution and Ecology of Apoptosis in Protozoan Parasites
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002320
Research Articles
Eurasian-Origin Gene Segments Contribute to the Transmissibility, Aerosol Release, and Morphology of the 2009 Pandemic H1N1 Influenza Virus
PLOS Pathogens: published December 29, 2011 | https://doi.org/10.1371/journal.ppat.1002443
A Novel Human Cytomegalovirus Locus Modulates Cell Type-Specific Outcomes of Infection
PLOS Pathogens: published December 29, 2011 | https://doi.org/10.1371/journal.ppat.1002444
Eight RGS and RGS-like Proteins Orchestrate Growth, Differentiation, and Pathogenicity of Magnaporthe oryzae
PLOS Pathogens: published December 29, 2011 | https://doi.org/10.1371/journal.ppat.1002450
Related Articles
Inflammasome-dependent Pyroptosis and IL-18 Protect against Burkholderia pseudomallei Lung Infection while IL-1β Is Deleterious
PLOS Pathogens: published December 29, 2011 | https://doi.org/10.1371/journal.ppat.1002452
Inflammatory Monocytes and Neutrophils Are Licensed to Kill during Memory Responses In Vivo
PLOS Pathogens: published December 29, 2011 | https://doi.org/10.1371/journal.ppat.1002457
Allelic Variation on Murine Chromosome 11 Modifies Host Inflammatory Responses and Resistance to Bacillus anthracis
PLOS Pathogens: published December 29, 2011 | https://doi.org/10.1371/journal.ppat.1002469
Identification and Characterization of a Novel Non-Structural Protein of Bluetongue Virus
PLOS Pathogens: published December 29, 2011 | https://doi.org/10.1371/journal.ppat.1002477
Prion Uptake in the Gut: Identification of the First Uptake and Replication Sites
PLOS Pathogens: published December 22, 2011 | https://doi.org/10.1371/journal.ppat.1002449
Ce-Duox1/BLI-3 Generated Reactive Oxygen Species Trigger Protective SKN-1 Activity via p38 MAPK Signaling during Infection in C. elegans
PLOS Pathogens: published December 22, 2011 | https://doi.org/10.1371/journal.ppat.1002453
Helicobacter pylori versus the Host: Remodeling of the Bacterial Outer Membrane Is Required for Survival in the Gastric Mucosa
PLOS Pathogens: published December 22, 2011 | https://doi.org/10.1371/journal.ppat.1002454
Epstein-Barr Virus Evades CD4+ T Cell Responses in Lytic Cycle through BZLF1-mediated Downregulation of CD74 and the Cooperation of vBcl-2
PLOS Pathogens: published December 22, 2011 | https://doi.org/10.1371/journal.ppat.1002455
Engineered Anopheles Immunity to Plasmodium Infection
PLOS Pathogens: published December 22, 2011 | https://doi.org/10.1371/journal.ppat.1002458
Functional Analysis of the Kinome of the Wheat Scab Fungus Fusarium graminearum
PLOS Pathogens: published December 22, 2011 | https://doi.org/10.1371/journal.ppat.1002460
CNS Recruitment of CD8+ T Lymphocytes Specific for a Peripheral Virus Infection Triggers Neuropathogenesis during Polymicrobial Challenge
PLOS Pathogens: published December 22, 2011 | https://doi.org/10.1371/journal.ppat.1002462
Cryo Electron Tomography of Herpes Simplex Virus during Axonal Transport and Secondary Envelopment in Primary Neurons
PLOS Pathogens: published December 15, 2011 | https://doi.org/10.1371/journal.ppat.1002406
Pathogenesis and Host Response in Syrian Hamsters following Intranasal Infection with Andes Virus
PLOS Pathogens: published December 15, 2011 | https://doi.org/10.1371/journal.ppat.1002426
Structural Elucidation and Functional Characterization of the Hyaloperonospora arabidopsidis Effector Protein ATR13
PLOS Pathogens: published December 15, 2011 | https://doi.org/10.1371/journal.ppat.1002428
Inhibition of Apoptosis and NF-κB Activation by Vaccinia Protein N1 Occur via Distinct Binding Surfaces and Make Different Contributions to Virulence
PLOS Pathogens: published December 15, 2011 | https://doi.org/10.1371/journal.ppat.1002430
Association of Activating KIR Copy Number Variation of NK Cells with Containment of SIV Replication in Rhesus Monkeys
PLOS Pathogens: published December 15, 2011 | https://doi.org/10.1371/journal.ppat.1002436
Related Articles
Synergistic Roles of Eukaryotic Translation Elongation Factors 1Bγ and 1A in Stimulation of Tombusvirus Minus-Strand Synthesis
PLOS Pathogens: published December 15, 2011 | https://doi.org/10.1371/journal.ppat.1002438
Fungal Virulence and Development Is Regulated by Alternative Pre-mRNA 3′End Processing in Magnaporthe oryzae
PLOS Pathogens: published December 15, 2011 | https://doi.org/10.1371/journal.ppat.1002441
Nef Decreases HIV-1 Sensitivity to Neutralizing Antibodies that Target the Membrane-proximal External Region of TMgp41
PLOS Pathogens: published December 15, 2011 | https://doi.org/10.1371/journal.ppat.1002442
The Membrane Fusion Step of Vaccinia Virus Entry Is Cooperatively Mediated by Multiple Viral Proteins and Host Cell Components
PLOS Pathogens: published December 15, 2011 | https://doi.org/10.1371/journal.ppat.1002446
Granzyme B Inhibits Vaccinia Virus Production through Proteolytic Cleavage of Eukaryotic Initiation Factor 4 Gamma 3
PLOS Pathogens: published December 15, 2011 | https://doi.org/10.1371/journal.ppat.1002447
Juxtamembrane Shedding of Plasmodium falciparum AMA1 Is Sequence Independent and Essential, and Helps Evade Invasion-Inhibitory Antibodies
PLOS Pathogens: published December 15, 2011 | https://doi.org/10.1371/journal.ppat.1002448
Related Articles
Quantitative Multicolor Super-Resolution Microscopy Reveals Tetherin HIV-1 Interaction
PLOS Pathogens: published December 15, 2011 | https://doi.org/10.1371/journal.ppat.1002456
A Receptor-based Switch that Regulates Anthrax Toxin Pore Formation
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002354
A Novel Role for the NLRC4 Inflammasome in Mucosal Defenses against the Fungal Pathogen Candida albicans
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002379
Kaposi's Sarcoma Herpesvirus microRNAs Target Caspase 3 and Regulate Apoptosis
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002405
A Host Small GTP-binding Protein ARL8 Plays Crucial Roles in Tobamovirus RNA Replication
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002409
Deficiency of a Niemann-Pick, Type C1-related Protein in Toxoplasma Is Associated with Multiple Lipidoses and Increased Pathogenicity
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002410
Toward an Integrated Model of Capsule Regulation in Cryptococcus neoformans
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002411
Sequential Adaptive Mutations Enhance Efficient Vector Switching by Chikungunya Virus and Its Epidemic Emergence
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002412
Norovirus Regulation of the Innate Immune Response and Apoptosis Occurs via the Product of the Alternative Open Reading Frame 4
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002413
Wolbachia Symbiont Infections Induce Strong Cytoplasmic Incompatibility in the Tsetse Fly Glossina morsitans
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002415
Epstein-Barr Virus Nuclear Antigen 3C Stabilizes Gemin3 to Block p53-mediated Apoptosis
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002418
Related Articles
IRGM Is a Common Target of RNA Viruses that Subvert the Autophagy Network
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002422
Multifaceted Regulation of Translational Readthrough by RNA Replication Elements in a Tombusvirus
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002423
Latent KSHV Infection of Endothelial Cells Induces Integrin Beta3 to Activate Angiogenic Phenotypes
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002424
SAMHD1-Deficient CD14+ Cells from Individuals with Aicardi-Goutières Syndrome Are Highly Susceptible to HIV-1 Infection
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002425
Controlling Viral Immuno-Inflammatory Lesions by Modulating Aryl Hydrocarbon Receptor Signaling
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002427
Sialidases Affect the Host Cell Adherence and Epsilon Toxin-Induced Cytotoxicity of Clostridium perfringens Type D Strain CN3718
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002429
Role of Permissive Neuraminidase Mutations in Influenza A/Brisbane/59/2007-like (H1N1) Viruses
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002431
The Human Cytomegalovirus UL11 Protein Interacts with the Receptor Tyrosine Phosphatase CD45, Resulting in Functional Paralysis of T Cells
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002432
SARS Coronavirus nsp1 Protein Induces Template-Dependent Endonucleolytic Cleavage of mRNAs: Viral mRNAs Are Resistant to nsp1-Induced RNA Cleavage
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002433
Targeting of Mycobacterium tuberculosis Heparin-Binding Hemagglutinin to Mitochondria in Macrophages
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002435
HIV-1 Capsid-Cyclophilin Interactions Determine Nuclear Import Pathway, Integration Targeting and Replication Efficiency
PLOS Pathogens: published December 8, 2011 | https://doi.org/10.1371/journal.ppat.1002439
Feeding Cells Induced by Phytoparasitic Nematodes Require γ-Tubulin Ring Complex for Microtubule Reorganization
PLOS Pathogens: published December 1, 2011 | https://doi.org/10.1371/journal.ppat.1002343
Neonatal CD8 T-cell Hierarchy Is Distinct from Adults and Is Influenced by Intrinsic T cell Properties in Respiratory Syncytial Virus Infected Mice
PLOS Pathogens: published December 1, 2011 | https://doi.org/10.1371/journal.ppat.1002377
Two Novel Transcriptional Regulators Are Essential for Infection-related Morphogenesis and Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae
PLOS Pathogens: published December 1, 2011 | https://doi.org/10.1371/journal.ppat.1002385
Longevity and Composition of Cellular Immune Responses Following Experimental Plasmodium falciparum Malaria Infection in Humans
PLOS Pathogens: published December 1, 2011 | https://doi.org/10.1371/journal.ppat.1002389
Chikungunya Virus Neutralization Antigens and Direct Cell-to-Cell Transmission Are Revealed by Human Antibody-Escape Mutants
PLOS Pathogens: published December 1, 2011 | https://doi.org/10.1371/journal.ppat.1002390
A Systematic Screen to Discover and Analyze Apicoplast Proteins Identifies a Conserved and Essential Protein Import Factor
PLOS Pathogens: published December 1, 2011 | https://doi.org/10.1371/journal.ppat.1002392
Acquisition of Pneumococci Specific Effector and Regulatory Cd4+ T Cells Localising within Human Upper Respiratory-Tract Mucosal Lymphoid Tissue
PLOS Pathogens: published December 1, 2011 | https://doi.org/10.1371/journal.ppat.1002396
Acid Stability of the Hemagglutinin Protein Regulates H5N1 Influenza Virus Pathogenicity
PLOS Pathogens: published December 1, 2011 | https://doi.org/10.1371/journal.ppat.1002398
Follicular Dendritic Cell-Specific Prion Protein (PrPc) Expression Alone Is Sufficient to Sustain Prion Infection in the Spleen
PLOS Pathogens: published December 1, 2011 | https://doi.org/10.1371/journal.ppat.1002402
Late Repression of NF-κB Activity by Invasive but Not Non-Invasive Meningococcal Isolates Is Required to Display Apoptosis of Epithelial Cells
PLOS Pathogens: published December 1, 2011 | https://doi.org/10.1371/journal.ppat.1002403
A Temporal Role Of Type I Interferon Signaling in CD8+ T Cell Maturation during Acute West Nile Virus Infection
PLOS Pathogens: published December 1, 2011 | https://doi.org/10.1371/journal.ppat.1002407
Computational and Biochemical Analysis of the Xanthomonas Effector AvrBs2 and Its Role in the Modulation of Xanthomonas Type Three Effector Delivery
PLOS Pathogens: published December 1, 2011 | https://doi.org/10.1371/journal.ppat.1002408
The Enteropathogenic E. coli (EPEC) Tir Effector Inhibits NF-κB Activity by Targeting TNFα Receptor-Associated Factors
PLOS Pathogens: published December 1, 2011 | https://doi.org/10.1371/journal.ppat.1002414
Autophagy Protein Atg3 is Essential for Maintaining Mitochondrial Integrity and for Normal Intracellular Development of Toxoplasma gondii Tachyzoites
PLOS Pathogens: published December 1, 2011 | https://doi.org/10.1371/journal.ppat.1002416
Genesis of Mammalian Prions: From Non-infectious Amyloid Fibrils to a Transmissible Prion Disease
PLOS Pathogens: published December 1, 2011 | https://doi.org/10.1371/journal.ppat.1002419
Polar Flagellar Biosynthesis and a Regulator of Flagellar Number Influence Spatial Parameters of Cell Division in Campylobacter jejuni
PLOS Pathogens: published December 1, 2011 | https://doi.org/10.1371/journal.ppat.1002420