Figures
Destruction of blood vessels by an hemorrhagic virus and spread to adjacent tissues.
This fli1:GFP transgenic zebrafish larva has been inoculated with Infectious Hematopoietic Necrosis Virus (IHNV), fixed 24 hours later, processed for whole-mount immunohistochemistry, and imaged with a confocal microscope to reveal virus-infected cells (anti-G-IHNV antibody, red), endothelial cells (GFP, green), and nuclei (blue). Infection of several vessels can be observed, especially at the top of the head, where loss of GFP expression has occurred, as well as infection of neighboring tissue. Although at this stage the larva does not display obvious macroscopic signs of disease, it is already doomed (see Ludwig et al., doi:10.1371/journal.ppat.1001269).
Image Credit: Nuno Palha, Institut Pasteur
Citation: (2011) PLoS Pathogens Issue Image | Vol. 7(2) February 2011. PLoS Pathog 7(2): ev07.i02. https://doi.org/10.1371/image.ppat.v07.i02
Published: February 24, 2011
Copyright: © 2011 Palha. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This fli1:GFP transgenic zebrafish larva has been inoculated with Infectious Hematopoietic Necrosis Virus (IHNV), fixed 24 hours later, processed for whole-mount immunohistochemistry, and imaged with a confocal microscope to reveal virus-infected cells (anti-G-IHNV antibody, red), endothelial cells (GFP, green), and nuclei (blue). Infection of several vessels can be observed, especially at the top of the head, where loss of GFP expression has occurred, as well as infection of neighboring tissue. Although at this stage the larva does not display obvious macroscopic signs of disease, it is already doomed (see Ludwig et al., doi:10.1371/journal.ppat.1001269).
Image Credit: Nuno Palha, Institut Pasteur