Evaluating the Sensitivity of Mycobacterium tuberculosis to Biotin Deprivation Using Regulated Gene Expression
Figure 5
Growth of Mtb bioA TetON-5 in biotin free liquid media and of all mutants in mice without doxy.
(A) Growth of wt and bioA TetON-5 in biotin-free Sauton's medium with and without atc. Squares represent data for wt, triangles represent data for bioA TetON-5. Open symbols indicate data from medium without atc, closed symbols represent data from medium containing atc. Data are from individual cultures and representative of at least two independent experiments. (B) Growth of wt, ΔbioA and the different bioA TetON mutants in mouse lungs. Closed symbols represent CFUs from day 1 post infection, open symbols represent data from day 21. The dotted line indicates the limit of detection (which was lower for wt and ΔbioA than for the TetON mutants due to the different amounts of lung homogenates that were plated). Each symbol represents CFUs obtained from one mouse.