Rapid End-Point Quantitation of Prion Seeding Activity with Sensitivity Comparable to Bioassays

Jason M. Wilham; Christina D. Orrú; Richard A. Bessen; Ryuichiro Atarashi; Kazunori Sano; Brent Race; Kimberly D. Meade-White; Lara M. Taubner; Andrew Timmes; Byron Caughey

doi:10.1371/journal.ppat.1001217

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Peer-reviewed

Research Article

Rapid End-Point Quantitation of Prion Seeding Activity with Sensitivity Comparable to Bioassays

Jason M. Wilham,

Affiliation Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America
⨯
Christina D. Orrú,

Affiliations Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America, Department of Biomedical Sciences and Technologies, University of Cagliari, Monserrato, Italy
⨯
Richard A. Bessen,

Affiliation Veterinary Molecular Biology, Montana State University, Bozeman, Montana, United States of America
⨯
Ryuichiro Atarashi,

Affiliation Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Kyushu, Japan
⨯
Kazunori Sano,

Affiliation Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Kyushu, Japan
⨯
Brent Race,

Affiliation Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America
⨯
Kimberly D. Meade-White,

Affiliation Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America
⨯
Lara M. Taubner,

Affiliation Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America
⨯
Andrew Timmes,

Affiliation Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America
⨯
Byron Caughey

* E-mail: BCAUGHEY@niaid.nih.gov

Affiliation Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, Hamilton, Montana, United States of America
⨯

Rapid End-Point Quantitation of Prion Seeding Activity with Sensitivity Comparable to Bioassays

Jason M. Wilham,
Christina D. Orrú,
Richard A. Bessen,
Ryuichiro Atarashi,
Kazunori Sano,
Brent Race,
Kimberly D. Meade-White,
Lara M. Taubner,
Andrew Timmes,
Byron Caughey

Published: December 2, 2010
https://doi.org/10.1371/journal.ppat.1001217

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