About the Authors

Christina D. Orrú

Contributed equally to this work with: Christina D. Orrú, Bradley R. Groveman

Affiliation Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, Montana, United States of America

Bradley R. Groveman

Contributed equally to this work with: Christina D. Orrú, Bradley R. Groveman

Affiliation Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, Montana, United States of America

Lynne D. Raymond

Affiliation Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, Montana, United States of America

Andrew G. Hughson

Affiliation Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, Montana, United States of America

Romolo Nonno

Affiliation Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Rome, Italy

Wenquan Zou

Affiliation Department of Pathology, Case Western Reserve University, Cleveland, Ohio, United States of America

Bernardino Ghetti

Affiliation Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, Indiana, United States of America

Pierluigi Gambetti

Affiliation Department of Pathology, Case Western Reserve University, Cleveland, Ohio, United States of America

Byron Caughey

bcaughey@nih.gov

Affiliation Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, Montana, United States of America

Competing Interests

I have read the journal's policy and the authors of this manuscript have the following competing interests: We are preparing a patent application to cover novel RT-QuIC applications described in this study. This does not alter our adherence to all PLOS policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: BC CDO BRG. Performed the experiments: CDO BRG. Analyzed the data: CDO BRG BC. Contributed reagents/materials/analysis tools: LDR AGH RN WZ BG PG. Wrote the paper: BC CDO BRG LDR AGH.