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Vector cloning

Superoxide dismutase VPA1514 in Vibrio parahaemolyticus protects against environmental stresses

Paula Hsu, Hin-chung Wong, Chung-Tao Tang

published 14 Aug 2025

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Different results despite high homology: Comparative expression of human and murine DNase1 in Pichia pastoris

Jan-Ole Krischek, Hans Georg Mannherz, Markus Napirei

published 29 Apr 2025

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Revolutionizing Molecular cloning: Introducing FastCloneAssist, a Streamlined Python tool for optimizing primer design in restriction & ligation-independent PCR cloning

Pradip Kumar Singh, Michael S. Donnenberg

published 13 Mar 2025

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DAPE cloning with modified primers for producing designated lengths of 3’ single-stranded ends in PCR products

Seoee Lee, Hyunyoung Kim, Aqsa, Kwangjin Jeung, Minho Won, Hyunju Ro

published 13 Feb 2025

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Recombinant cyclin B-Cdk1-Suc1 capable of multi-site mitotic phosphorylation in vitro

Keishi Shintomi, Yuki Masahara-Negishi, Masami Shima, Shoji Tane, Tatsuya Hirano

published 25 Mar 2024

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A comprehensive, improved protocol for generating common bean (Phaseolus vulgaris L.) transgenic hairy roots and their use in reverse-genetics studies

Ronal Pacheco, Georgina Estrada-Navarrete, Jorge Solis-Miranda, Noreide Nava, MA Juárez-Verdayes, Yolanda Ortega-Ortega, Carmen Quinto

published 21 Feb 2024

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A simple and efficient protocol for generating transgenic hairy roots using Agrobacterium rhizogenes

Shaun Ferguson, Nikolaj B. Abel, Dugald Reid, Lene H. Madsen, Thi-Bich Luu, Kasper R. Andersen, Jens Stougaard, Simona Radutoiu

published 01 Nov 2023

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The chicken chorioallantoic membrane model for isolation of CRISPR/cas9-based HSV-1 mutant expressing tumor suppressor p53

Mishar Kelishadi, Hosein Shahsavarani, Alijan Tabarraei, Mohammad Ali Shokrgozar, Ladan Teimoori-Toolabi, Kayhan Azadmanesh

published 20 Oct 2023

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Improved USER cloning for TALE assembly and its application to base editing

Jizeng Zhou, Jiaowei Wang, Fangbing Chen, Zhenpeng Zhuang, Min Chen, Yang Yang, Xian Luo, Chengcheng Tang, Xiaoqing Zhou, Yue Chi, Jinling Wang, Yu He, Kun Zhang, Qingjian Zou

published 04 Aug 2023

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High-throughput FastCloning technology: A low-cost method for parallel cloning

Hua Jiang, Fan Meng, Deren Lu, Yanjuan Chen, Guilin Luo, Yuejun Chen, Jun Chen, Cheng Chen, Xi Zhang, Dan Su

published 09 Sep 2022

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Characterization of an intracellular humanized single-chain antibody to matrix protein (M1) of H5N1 virus

He Sun, Guangmou Wu, Jiyuan Zhang, Yu Wang, Yue Qiu, Hongyang Man, Guoli Zhang, Zehong Li, Yuhuan Yue, Yuan Tian

published 31 Mar 2022

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MICA-G129R: A bifunctional fusion protein increases PRLR-positive breast cancer cell death in co-culture with natural killer cells

Hui Ding, Garrett W. Buzzard, Sisi Huang, Michael G. Sehorn, R. Kenneth Marcus, Yanzhang Wei

published 02 Jun 2021

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A bicistronic vector backbone for rapid seamless cloning and chimerization of αβT-cell receptor sequences

Korbinian N. Kropp, Tim J. Schäufele, Martina Fatho, Michael Volkmar, Roland Conradi, Matthias Theobald, Thomas Wölfel, Catherine Wölfel

published 09 Sep 2020

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