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Protein engineering

A High-Throughput Colorimetric Screening Assay for Terpene Synthase Activity Based on Substrate Consumption

Maiko Furubayashi, Mayu Ikezumi, Jun Kajiwara, Miki Iwasaki, Akira Fujii, Ling Li, Kyoichi Saito, Daisuke Umeno

published 28 Mar 2014

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Mutagenic Organized Recombination Process by Homologous In Vivo Grouping (MORPHING) for Directed Enzyme Evolution

David Gonzalez-Perez, Patricia Molina-Espeja, Eva Garcia-Ruiz, Miguel Alcalde

published 10 Mar 2014

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Seven N-terminal Residues of a Thermophilic Xylanase Are Sufficient to Confer Hyperthermostability on Its Mesophilic Counterpart

Shan Zhang, Yongzhi He, Haiying Yu, Zhiyang Dong

published 30 Jan 2014

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Improving Kinetic or Thermodynamic Stability of an Azoreductase by Directed Evolution

Vânia Brissos, Nádia Gonçalves, Eduardo P. Melo, Lígia O. Martins

published 27 Jan 2014

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Tracking Molecular Recognition at the Atomic Level with a New Protein Scaffold Based on the OB-Fold

John D. Steemson, Matthias Baake, Jasna Rakonjac, Vickery L. Arcus, Mark T. Liddament

published 20 Jan 2014

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Improving Glyphosate Oxidation Activity of Glycine Oxidase from Bacillus cereus by Directed Evolution

Tao Zhan, Kai Zhang, Yangyan Chen, Yongjun Lin, Gaobing Wu, Lili Zhang, Pei Yao, Zongze Shao, Ziduo Liu

published 05 Nov 2013

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Directed Evolution of Aminoglycoside Phosphotransferase (3′) Type IIIa Variants That Inactivate Amikacin but Impose Significant Fitness Costs

Joseph R. Kramer, Ichiro Matsumura

published 18 Oct 2013

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Mechanism of Protein Kinetic Stabilization by Engineered Disulfide Crosslinks

Inmaculada Sanchez-Romero, Antonio Ariza, Keith S. Wilson, Michael Skjøt, Jesper Vind, Leonardo De Maria, Lars K. Skov, Jose M. Sanchez-Ruiz

published 30 Jul 2013

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Fitness Loss and Library Size Determination in Saturation Mutagenesis

Yuval Nov

published 03 Jul 2013

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Engineered Monoclonal Antibody with Novel Antigen-Sweeping Activity In Vivo

Tomoyuki Igawa, Atsuhiko Maeda, Kenta Haraya, Tatsuhiko Tachibana, Yuki Iwayanagi, Futa Mimoto, Yoshinobu Higuchi, Shinya Ishii, Shigero Tamba, Naoka Hironiwa, Kozue Nagano, Tetsuya Wakabayashi, Hiroyuki Tsunoda, Kunihiro Hattori

published 07 May 2013

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Engineering Agatoxin, a Cystine-Knot Peptide from Spider Venom, as a Molecular Probe for In Vivo Tumor Imaging

Sarah J. Moore, Cheuk Lun Leung, Heidi K. Norton, Jennifer R. Cochran

published 03 Apr 2013

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Identification and Multidimensional Optimization of an Asymmetric Bispecific IgG Antibody Mimicking the Function of Factor VIII Cofactor Activity

Zenjiro Sampei, Tomoyuki Igawa, Tetsuhiro Soeda, Yukiko Okuyama-Nishida, Chifumi Moriyama, Tetsuya Wakabayashi, Eriko Tanaka, Atsushi Muto, Tetsuo Kojima, Takehisa Kitazawa, Kazutaka Yoshihashi, Aya Harada, Miho Funaki, Kenta Haraya, Tatsuhiko Tachibana, Sachiyo Suzuki, Keiko Esaki, Yoshiaki Nabuchi, Kunihiro Hattori

published 28 Feb 2013

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Rational Engineering of a Human Anti-Dengue Antibody through Experimentally Validated Computational Docking

Luca Simonelli, Mattia Pedotti, Martina Beltramello, Elsa Livoti, Luigi Calzolai, Federica Sallusto, Antonio Lanzavecchia, Luca Varani

published 06 Feb 2013

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